Bacterial expression
Fast delivery;
HongTide make the gene optimization according to the target sequence you provide: mRNA and tRNA abundance, codon preference and other optimization.
A variety of expression vectors for selection:pET28a、pET30a、pCzn1、pSumo、pGEX、pMAL...
Gene optimization & synthesis
Plasmid + clone strain + expression strain
5 weekdays
1-3mg protein, purity > 85%
Protein expression & purification
10 weekdays
3-5mg protein, purity > 85%
10 weekdays
5-10mg protein, purity > 85%
10 weekdays
>10mg protein, purity > 85%
Protein expression & purification
15 weekdays
1-3mg protein, purity > 85%
HongTide make the gene optimization according to the target sequence you provide: mRNA and tRNA abundance, codon preference and other optimization.
Three vectors are constructed simultaneously: pCzn1, pET28a, pMAL; three expression strains, AE, TransB (DE) and BL21 (DE) 3plyss, are introduced.
Gene optimization & synthesis
Plasmid + clone strain + expression strain
Subcloning
1-2 weeks
Different expression plasmid & strain
Expression condition optimization
3-4 weeks
Pictures of expression analysis
Protein expression purification
5 weekdays
1-3mg protein, purity > 85%