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Home > Protein Expression > Bacterial expression

Bacterial expression

E.coli is the preferential one of bacterial systems for recombinant protein production and large scale fermentation as its advantages including fast rate of reproduction, ease of culture, and rich knowledge about its genetics. With abundant experience of soluble protein expression and production and protein refolding, HongTide can provide you high-quality expression service.
Five-Days Soluble Protein Rapid Delivery Package

Features:

  • Fast delivery;

  • HongTide make the gene optimization according to the target sequence you provide: mRNA and tRNA abundance, codon preference and other optimization.

  • A variety of expression vectors for selection：pET28a、pET30a、pCzn1、pSumo、pGEX、pMAL...

Service Content
Timeline
Deliverables

  • Gene optimization & synthesis

  • Plasmid + clone strain + expression strain

5 weekdays

  • 1-3mg protein, purity > 85%

  • Protein expression & purification

10 weekdays

  • 3-5mg protein, purity > 85%

10 weekdays

  • 5-10mg protein, purity > 85%

10 weekdays

  • >10mg protein, purity > 85%

  • Protein expression & purification

15 weekdays

  • 1-3mg protein, purity > 85%

The Supernatant Express Guaranteed Package

Features:

  • HongTide make the gene optimization according to the target sequence you provide: mRNA and tRNA abundance, codon preference and other optimization.

  • Three vectors are constructed simultaneously: pCzn1, pET28a, pMAL; three expression strains, AE, TransB (DE) and BL21 (DE) 3plyss, are introduced.

Service Content
Timeline
Deliverables

  • Gene optimization & synthesis

  • Plasmid + clone strain + expression strain

  • Subcloning

1-2 weeks

  • Different expression plasmid & strain

  • Expression condition optimization

3-4 weeks

  • Pictures of expression analysis

  • Protein expression purification

5 weekdays

  • 1-3mg protein, purity > 85%

  • >3mg protein, purity > 85%

Prokaryotic Portein Expression Validated Package

Verification Package A

Features: The service will be charge no matter whether the expression will success using the expression carrier provided by the customer.
Custom providing
Service Content
Timeline
1. >100ng non-polluting plasmid
2. Original plasmid map and sequence file
3. Commercial sequencing report of plasmids after insertion of genes
Re-transform AE host bacteria with the plasmid provided by the client to verify the expression of the target protein and analyze the expression pattern of the target protein (supernatant or inclusion body expression)
2-4 days

Verification Package B

Features: Subclone plasmid provided by client to the characteristic expression vector, transforms the matched strain. The service will charge after the expression is successful; if expression fails, we only charge the cost of Subcloning.
Custom providing
Service Content
Timeline
1. >100ng non-polluting plasmid
2. Original plasmid map and sequence file
3. Commercial sequencing report of plasmids after insertion of genes
Use plasmid to transform the low temperature AE host bacteria, verify the expression, and analyze the expression form of the target protein (supernatant or inclusion body)
1-2 weeks